Unlike Illumina and 454, Ion torrent and Ion proton sequencing do not make use of optical signals. Instead, they exploit the fact that addition of a dNTP to a DNA polymer releases an H + ion. As in other kinds of NGS, the input DNA or RNA is fragmented, this time ~200bp. Adaptors are added and one molecule is placed onto a bead.

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 · The reduction of iron oxides takes place according to the following chemical equations: (1) F e 2 O 3 + 3 C O → 2 F e + 3 C O 2 (2) F e 2 O 3 + 3 H 2 → 2 F e + 3 H 2 O In contrast to the blast furnace process, the direct-reduced iron process operates at temperatures of up to 1000 °C. The iron ores are therefore not melted!

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 · How do you use it? To use the Ion Torrent sequencer, prepare genomic DNA like you would for any other sequencer (fragment DNA, ligate to adapters and clonally amplify your adapter-ligated libraries onto beads). DNA polymerase and primers are bound to templates and pipetted into the loading port.

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 · Ion torrent： Follow Cycle： "Follow"：dNTP(T, A, C, or G),； "Cycle"：4dNTP, …

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 · Direct reduced iron (DRI) process. In addition to the process route via the blast furnace process, there is another option for extracting crude steel from iron ores using the so-called direct reduced iron (DRI) process (secondary route).The starting point for the efforts to find an alternative to the blast furnace process was the relatively high process …

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 · The sequencing run is started and runs according to the plan prepared before the run. Thermo Fisher's Ion Torrent uses semiconductor sequencing technology. Nucleotides are flowed over the chip one at a time. If the nucleotide is incorporated, a hydrogen ion is released. This release of hydrogen decreases the pH of the liquid surrounding the ISP.

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 · Screening and diverting fine particles significantly increases the performance of the crusher. 2. Crushing and stock piling. This step involves breaking up the iron ore to obtain an F 80 grind size before it can be …

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is as simple as it is fast. Ion Torrent™ technology directly translates chemically encoded information (A, C, G, T) into digital information (0, 1) on a semiconductor chip. This …

DRI production. Direct reduction of iron is the removal of oxygen from iron ore or other iron bearing materials in the solid state, i.e. without melting, as in the blast furnace. The reducing agents are carbon monoxide and …

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